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氢氧化钾如何测定蛋白质的溶解度

发布来源:http://www.jinhao360.com    更新时间:2020-06-20 点击数:

氢氧化钾蛋白质溶解度可以反映蛋白质变性的情况。不同的蛋白质品种,质溶解度不同。蛋白质变性越大,蛋白质溶解度越小。下面就为大家详细讲解如何测定其溶解度。  

The solubility of KOH protein can reflect the denaturation of protein. Different protein varieties have different solubility. The larger the denaturation of protein, the smaller the solubility of protein. Next, I will explain how to determine its solubility in detail.
1、用一定浓度的氢氧化钾溶液提取试样中的可溶性蛋白质,在催化剂作用下用浓硫酸将提取液中可溶性蛋白质的氮转化为硫酸铵。加入强碱进行蒸馏使氨逸出,用硼酸吸收后,再用盐酸滴定测出试样中可溶性蛋白质含量;同时,测定原始试样中粗蛋白质含量,计算出试样的蛋白溶解度。
1. A certain concentration of potassium hydroxide solution was used to extract the soluble protein in the sample. Under the action of catalyst, concentrated sulfuric acid was used to convert the nitrogen of the soluble protein in the extract into ammonium sulfate. Add strong base for distillation to make ammonia escape, absorb with boric acid, and then titrate with hydrochloric acid to measure the soluble protein content in the sample; at the same time, measure the crude protein content in the original sample, and calculate the protein solubility of the sample.
2、实验试剂
2. Experimental reagent
1)氢氧化钾(分析纯),无水硫酸钾、五水硫酸铜、氢氧化钠、硼酸、甲基红、溴甲酚绿、硫酸铵;
1) Potassium hydroxide (analytical pure), anhydrous potassium sulfate, copper sulfate pentahydrate, sodium hydroxide, boric acid, methyl red, bromocresol green, ammonium sulfate;
2)浓硫酸、盐酸(分析纯)、百分之九十五乙醇、蒸馏水。
2) Concentrated sulfuric acid, hydrochloric acid (analytical pure), 95% ethanol, distilled water.
3、仪器和设备
3. Instruments and equipment
1)感量为0.0001g分析天平;
1) The sensitivity is 0.0001g analytical balance;
2)磁力搅拌器;
2) Magnetic stirrer;
3)离心机(带离心管),转速为2700r/min以上;
3) Centrifuge (with centrifuge tube), rotating speed is more than 2700r / min;
4)样品粉碎机;
4) Sample crusher;
5)60目分析筛;
5) 60 mesh analysis screen;
6)电炉。
6) Electric furnace.
通过以上文中讲解的内容,大家可以了解到这种氢氧化钾蛋白质溶液的溶解度是如何测定的了。那么,大家在以后的操作使用中,不妨采取这种方法进行测定,更加方便、有效。
Through the content explained above, you can understand how the solubility of this potassium hydroxide protein solution is determined. Then, in the future operation and use, we might as well adopt this method for determination, which is more convenient and effective.

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